Background: Currently the prevalence of diabetes mellitus is increased alarmingly in low andrnmiddle income countries. Concurrently the drug resistant S. aureus nasal carriage rate is rnincreasing that may serve as a source of further infections in Diabetes mellitus patients. There isrnno study conducted on nasal carriage rate of S.aureus and Methicillin resistant S.aureus (MRSA)rnamong diabetes patients in Ethiopia. rnObjective: The aim of this study was to assess the rate of nasal carriage of S.aureus and MRSArnand associated factors among diabetic patients at Tikur Anbessa Specialized Hospital Diabetic rnClinic, Addis Ababa, Ethiopia from January to June, 2018.rnMethods: A cross-sectional study was conducted from January to June, 2018. A total of 422rndiabetic patients were recruited at TASH Diabetic Clinic. Nasal swab from the anterior nares was rncollected, and culture on both Manitol salt agar and blood agar. Antimicrobial susceptibilityrntesting was done using disc diffusion method on Muller Hinton agar. Susceptibility to methicillin rnwas phenotypically determined based on resistance of isolates to cefoxitin. Data was entered andrnanalyzed using SPSS software version 20. Binary logistic regression analysis was used to assessrnthe association of socio-demographic and associated factors for S.aureus culture isolates. P- value rn< 0.05 was considered as statistically significant. rnResults: The overall prevalence of S.aureus in the study was 47/422(11.1%). Majority of the S.rnaureus isolates were resistant to penicillin 46(97.8) on the other hand, all of the S. aureus isolatesrnwere sensitive to clindamycin. Multidrug resistance were shown in 4(6.2%) of the isolates. Of rnthese, one MRSA was isolated from retire man who had uncontrolled glucose level with Type IIrnDM despite insulin treatment and had foot wound. S.aureus isolation rate was significantlyrnhigher among DM patients who currently used combination of insulin and oral drug for diabetesrnmellitus treatment (odds ratio [AOR], 3.985, 95% CI, 1.270-12.437; p