Abstract rnBackground: Tuberculosis is an infectious disease caused mainly by the Mycobacteriumrntuberculosis and remains a major cause of ill Health and death worldwide. Ethiopia is one of thern30 highest TB burden countries in the world, Federal Ministry of Health of Ethiopia isrnimplementing TB Prevention and control program at all level of the Health facility to reach 70%rndetection rate and to achieve sustainable quality TB service. The performance of TB smearrnmicroscopy quality of slide had no improvement in order to mitigate this the laboratories shallrnparticipate on continuous EQA and review the feedback by taking corrective action forrnsustainable performance. rnObjective: To assess Feedback quality & utilization on TB microscopy EQA performance fromrnFeb - March 2020. rnMethod: Institutional based retrospective and prospective cross sectional study design werernconducted at 47 public Health centers which were selected by simple random sampling methodrnfrom ten sub cities in Addis Ababa, Ethiopia. Data collection was conducted using semistructuredrnrnquestionnaire to assess feedback quality & utilization on TB microscopy EQArnperformance from February- March 2020. The data was entered by EPI Info version 7 andrnanalyzed by Microsoft Excel, SPSS version 23 and narrative form. rnResult: The overall TB EQA feedback utilization of Addis Ababa public Health centers showedrnthat only 15.9% of the facilities were using the feedback for improvement by identifying rootrncause, taking corrective action and follows the effectiveness. On TB microscopy blindedrnrechecking program feedback which was given for public Health centers from EQA centers havernonly 6.8% quality that is timely, accurate and reliable. Most of the laboratories have a challengernon competency, lack of commitment and lack of trained laboratory personnel on TB microscopyrnand quality management system. rnConclusion: The performance of Public Health facility laboratories on TB microscopy EQArnquality of slide was poor in Addis Ababa, due to the facilities were not utilizing the feedbackrneffectively for improving their achievement. And also regarding a quality feedback EQA centersrnhad gap on timely distributing the feedback for Health facilities and overall feedback content.