Bacteriological Profile Antimicrobial Resistance And Outcome Of Neonatal Sepsis Among Patients At St.paul Hospital Millennium Medical College Addis Ababa Ethiopia

Background: Neonatal septicemia is the presence of clinically associated bacteria or fungi in thernblood of neonate’s .It causes high morbidity and mortality worldwide especially in developingrncountry. rnObjectives: The study was designed to assess the bacteriological profile, antimicrobial resistancernand outcome of neonatal sepsis at St. Paul’s Hospital Millennium Medical College.rnMethods: A hospital based cross sectional study design was conducted at St. Paul’s HospitalrnMillennium Medical College from March 2020 to July 2020 G.C. Socio-demographic andrnclinical data was collected from each patient. 1-2ml of blood was drawn aseptically andrninoculated at bedside on Trypto Soya Broth. Gram stain was performed and subcluturing wasrndone every other day on blood agar, chocolate agar and Mac Conkey agar plates. For speciesrnidentification, colony characteristics and biochemical tests are used if the culture is positive. Allrnthe isolates was tested for susceptibility test by using Kirby-Bauer’s disk diffusion method.rnOutcome of neonates was assessed using checklists. Data was encoded in to Microsoft Excel andrnanalyzed using Statistical Package for Social Sciences (SPSS) version 20 software. rnResults: Among 400 study populations, 84(21%) showed bacterial growth, 67 (79.8%) gramrnnegative and 17 (20.23%) gram positive bacteria. Commonly isolated organisms were Klebsiellarnspp 37 (44%), E. coli 19 (21.6%) and Coagulase negative Staphylococci 13 (15.47%) were thernleading causes of neonatal sepsis in our study. Antibiotic resistance was found in both Gramrnpositive and Gram negative bacteria. Ciprofloxacin and Amikacin were the most effectivernantibiotic for gram negative bacteria while for gram positive bacteria Vancomycin, Clindamycinrnand Ciprofloxacin were effective drugs. rnConclusion: Bacterial isolates were not giving response to first and second line antimicrobialsrnthat are utilized for the treatment of sepsis in babies who are in the first month of life. Antibioticsrnin the third line are moderately successful against isolated bacteria. High utilization rate ofrnantibiotics is the most important contributory factor for the development of AMR and continuousrnsurveillance is needed in order to keep national guidelines on antimicrobial therapy updated.

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