Background: Nowadays magnitude of Extended Spectrum Î²- Lactamases (ESBL) producingrnbacteria which cause urinary tract infection is the most worrying issue in the world. Thernoccurrence of ESBL producers especially in pregnant women can result in life threateningrncondition and morbidity for both the mother and the newborn due to unavailability of diagnosticrnmethod and very limited drug options for treatment of these pathogens in our country. Therefore,rnevidence regarding the magnitude of ESBL producers among pregnant women is demanding.rnObjectives: To determine Extended Spectrum Beta Lactamase in uropathogens andrnantimicrobial susceptibility pattern of Gram negative bacilli isolates among pregnant womenrnattending antenatal care in ALERT hospital, Addis Ababa, Ethiopia. rnMethods: A cross sectional study was conducted from July to September 2018 on a total of 177rnpregnant women with and without symptoms of urinary tract infection at ALERT hospital, AddisrnAbaba, Ethiopia. All urine samples were inoculated onto Cysteine Lactose Electrolyte Deficientrnmedium (CLED) and MacCkonkey agar. Colonies were counted to check the presence ofrnsignificant bacteriuria. Pure isolates of bacterial pathogen were characterized and identified atrnspecies level by colony morphology, gram-stain and standard biochemical procedures. All Gramrnnegative isolates were put into Muller-Hinton agar plates for antibiotic susceptibility test byrnKirby-Bauer disc diffusion technique. ESBL was detected using double disk synergy methods onrnMuller Hinton agar. The data were double entered into EPI INFO and analyzed using StatisticalrnPackage for Social Science (SPSS) version 26. rn Results: The overall prevalence of UTI among pregnant women was 14.7% (n=26/177). K.rnpneumoniae was the predominant bacterial etiologic agent of UTI (26.9% (n=7/26)). Thernprevalence of ESBL among gram negative isolates was 50% (n=6/12). Among ESBL producingrnisolates all (100%) were resistance to Amikacin, Gentamicin and Ceftriaxone while intermediaternlevel resistance rate of 66.7% was observed among Trimethoprim-sulphamethoxazole andrnCefixime. They were susceptible for some limited drugs and these were Nitrofurantoin (83.3%)rnand Chloramphenicol (83.3%).rnConclusions:- Majority of ESBL producing isolates exhibited co-resistance to other commonlyrnprescribed antibiotics. This indicates that the option of treatment for these pathogens rapidlyrndecreased from time to time which results serous life threating conditions especially in motherrnand newborn unless the appropriate measure is taken.