Hemoglobinopathies are inherited abnormalities of hemoglobin molecule that affect thernproduction, structure and function of Hb. Hb is the protein of the red blood cells that bindsrnoxygen reversibly and transport it from the lungs to the tissue and return carbon dioxide from therntissue back to the lungs. Hemoglobin genes are subject to mutations that alter the synthesis andrnstructure of the protein. These diseases are common lethal genetic defects in some part of thernworld. This study was undertaken to find out the hemoglobin profile of anemic patients attendingrnfollow-up clinics at the TASH. The study subjects included both genders, between 3 months torn68 years of age. Information was collected from the patients by a questionnaire designed to findrnout the relationship of hemoglobin variants with gender, age, family relation ship, etc. Bloodrnfilms were prepared to look for morphological appearances of red cells and also malariarnparasites. Out of 113 patients studied, only one was found to have malaria parasite on blood film.rn90 % of the morphologies revealed variable size and shape of the red blood cells. The CBCrnrevealed that the Hb of the patients ranged from 3.0 to 12.0 gm/dl and the MCV and MCH werern56.1-122.0 fl and 14.1-50.5pg respectively. The hemolysate that was subjected to agarose andrnNative-PAGE revealed that all the patients had HbA, HbF and HbA2 and three patients (2.65 %)rnof the participants had sickle hemoglobin band. There were six patients (5.3 %) with thick HbA2rnband. It is clear from this study that there is a need to conduct this type of research on largerrnsample using sensitive methods, including DNA analysis to investigate the prevalence andrndistribution of Hemoglobinopathies in Ethiopia