Blood And Tissue Enzyme Activities Of Gdh Ldh Index Of Glutathione And Oxidative Stress Among Breast Cancer Patients Attending Referal Hospitals Of Addis Ababa Ethiopia A Comparative Cross Sectional Study

Background: The exact cause of breast cancer is still unknown, however, it is a multifactorial andrndevastating disease. It is commonly diagnosed and second leading cause of death among womenrnglobally. Scholars are searching for an early marker for diagnosis, prognosis and potential targetrnfor treatment by looking it through many directions. One of the direction is its metabolism, allrntypes of cancer need high energy input and substrates via aberrant metabolism for their survival.rnTherefore, the present study focused on investigating and examining pathways of glucose andrnglutamate metabolism and oxidative stress in breast cancer patients via analysis of glutamaterndehydrogenase, lactate dehydrogenase, reduced and oxidized glutathione and the oxidative stressrnindex of breast cancer patients.rnMethodology: Comparative cross-sectional study was conducted from July, 2015 to May, 2017.rnA total of 54 (27 breast cancer patients and 27 control groups) participants were recruited. All werernage-sex matched and mean age of breast cancer patients were 44.93 (25–68), whereas mean agernof controls were 45.2 (24-66).rnResult: Catalytic activities of glutamate dehydrogenase, lactate dehydrogenase and oxidativernstress index were significantly increased both in serum (4.2mU/ml, 78.6mU/ml and 3.3:1rnrespectively) and cancerous tissues (1.4mU/ml), 111.7mU/ml and 2.15:1 respectively) of breastrncancer patients as compared to serum of control groups (3.15mU/ml, 30.4mU/ml and 2.05:1rnrespectively) and non-cancerous tissue of breast cancer patients (0.92mU/ml, 70.5mU/ml and 1.1:1rnrespectively) (P≤0.05). Correspondingly, ratios of reduced to oxidized glutathione werernsignificantly decreased both in serum (20:1) and cancerous tissues (23.5:1) of breast cancerrnpatients when compared to serum of control groups (104.5:1) and non-cancerous tissues of breastrncancer patient (70.9:1) (P≤0.05).rnConclusion: Catalytic activities of glutamate dehydrogenase and lactate dehydrogenase, and ratiosrnof total oxidative status to total antioxidant capacity were significantly increased among breastrncancer patients when compared to serum of control groups and non-cancerous tissues of breastrncancer patients. While, ratios of reduced to oxidized glutathione and total antioxidant capacity inrnserum and tumor tissues of breast cancer were significantly decreased as compared to serum ofrncontrol groups and normal tissues of breast cancer patients.rnKey Words: Glutamate Dehydrogenase, Lactate Dehydrogenase, Reduced Glutathione, OxidizedrnGlutathione, Total oxidative status and Total antioxidant capacity and breast cancer

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