Background: Biosafety is an important practice in all laboratory settings especially in developingrncountries where standard operating procedures are lacking. There is only limited information aboutrnbiosafety practices in government hospital laboratories in Addis Ababa.rnObjective: To assess the situation of biosafety practice and associated factors in clinical laboratoryrnworkers among government Hospitals in Addis Ababa, EthiopiarnMethod: Cross-sectional study design was conducted from March to May 2014; in all governmentrnHospitals of Addis Ababa. A total of 290 laboratory professionals were participated about theirrnbiosafety practice and its associated factorsrnusing structured questionnaire to assess the structural andrnfunctional aspects of Biosafety Practice of laboratories. Data were coded and entered by Epi Info andrnExported to SPSS V.20 for analysis. Bivariate and multivariate analysis were also used to identifyrnfactors that affect the outcome variable and to examine the effect of selected variables on laboratoryrnpersonnel practice toward biosafety practice by using Odds Ratio (OR) with a 95% Confidence Interval.rnP- Value less than 0.05 were taken as statistically significant. Variables that were found with a statisticallyrnsignificant association (p