Introduction: Septicemia defined as the presence of bacteria in the blood and is often associatedrnwith severe infections. It causes great impact in terms of mortality, morbidity and increased inrnhealthcare cost. There are many risk factors of septicemia among different patient groups.rnObjectives: The study was designed to assess bacterial profile, antibiotic susceptibility patternrnand associated risk factor of pediatrics septicemia at Zewuditu Memorial Hospital, Addis Ababa,rnEthiopia.rnMethods: A hospital based cross sectional study design; conducted at Zewuditu MemorialrnHospital from June 5, 2016 to March 8, 2017.A total of 309 study participants who were suspectedrnfor septicemia recruited .Socio-demographic and clinical data were collected from each patient.rnBlood was drawn aseptically and inoculated at bedside on Trypto Soya Broth. Gram stain wasrnperformed and the specimen was sub cultured every other day on to blood agar, chocolate agarrnand MacConkey agar plates. For culture positive; colony characteristics and Biochemical testsrnused for species identification. All the isolatestested for susceptibility by using Kirby-Bauerâ€™srndisk diffusion method. All data entered to EPIINFO version 3.5.1 and then exported to SPSSrnstatistical software version 20 for data analysis. Multiple Logistic regression analysis was used tornsee the association between dependent and independent variables.rnResults: Out of 309 samples, 113(36.5%) showed bacterial growth, 84(74.3%) gram positive andrn29(25.3%) gram negative bacteria. Commonly isolated organisms were Staphylococcus aureusrn57(50.4%),Coagulase negative Staphylococci25(22%) and Klebsiellapneumoniae21(18.5%).rnBirth weight, underlying chronic disease, congenital anomalies, neutrophil percentage, source ofrninfection and age of the pediatrics were associated with positive blood culture. Both Gramrnpositive and negative bacteria showed resistance for commonly prescribed antibiotics.rnClindamycin was the most effective antibiotic for gram positive bacteria while for gram negativernbacteria cefotetan and ceftraxion were effective drugs for gram negative bacteria.rnConclusion: The pattern of organism that cause pediatrics septicemia changes over time and inrngeographical location. High prevalence of antimicrobial resistance was noted in this study,rnespecially in gram negative bacteria. Moreover multi-drug resistance of the isolate wasrnsurprisingly high (89.3%).rnKeyword: septicemia, bacteriological profile, antimicrobial susceptibility pattern, blood culture