Seroepidemiological Study Of Brucellosis In Humans And Dairy Cattle In Addis Ababa

Sero-epidemiological study of brucellosis was conducted on occupationally exposed humans andrndairy cattle in Addis Ababa. A total of 336 human individuals were screened by Rose BengalrnPlate Test. Those found to be positive were further subjected to 2-MET. Similarly, 552 sexuallyrnmatured dairy cattle were screened for RBPT and positives were further subjected to SerumrnAgglutination Test and Complement Fixation Test. A simultaneous survey was conducted in thernfarms and Addis Ababa abattoir enterprise to investigate epidemiological factors. An over allrnseroprevalence of (16/336) 4.8% in humans and 10% (57/552) in dairy cattle was found, takingrnRBPT+ / 2MET+ in humans and RBPT+ / CFT+ in dairy cattle as a gold standard. A sensitivityrnand specificity of 88% and 94% in humans and 100% and 68.5% in dairy cattle was observed forrnRBPT. Where as SAT was 63.3% sensitive and 89.2% specific in dairy cattle. The risk factorsrncontributing to the occurrence of the disease in humans include occupation, sex, raw milk andrnmeat consumption, failure to use detergents after work, and unsafe handling of infectedrnmaterials. However, herd size and lack of knowledge about brucellosis are the major factors inrndairy cattle seropositive animals.rnKey words: Sero-epidemiological, sensitivity, specificity and risk factors

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