Evaluation And Optimization Of Agro-industrial Wastes For Conidial Production Of Metarhizium And Beauveria Isolates Under Solid State Fermentation

Two isolates of Beauveria and three isolates of Metarhizium were grown on differentrnagricultural wastes to evaluate their conidia production potential under SSF system.rnCoffee husk, Tea waste, Wheat bran and Vegetable wastes were used as substrates torndetermine maximum conidiation of the isolates. Among these vegetable wastes were thernbest medium to yield 5.77±1.53 (107), 5.80±0.72 (107), 4.44 ± 0.55(107) and 5.58 ± 0.66rn(107) conidia/gram of substrate under quantitative assessment for isolates (AUBI2,rnAUMI1, AUMI2 and AUMI3 respectively) at 60% moisture content. The highest conidiarncount record of AUBI1 6.34 ± 2.27 (107) conidia/gram was obtained at 60% moisturerncontent on wheat bran. It has been observed that two sample t-Test statistical analyses ofrnconidial yield of vegetable wastes was significantly different from 2mm and 4mm particlernsize of coffee husk used as substrates. The optimization for temperature indicated that allrnsubstrates supported maximum conidia yield with-in the range of temperature from 27 torn300rnC. Beauveria isolate AUBI1 was produced 11.63 ± 8.34 (107) conidia/gram ofrnsubstrate at coffee husk when treated at 3.5 pH value. The pH values used in the presentrnstudy for optimization best favored only for coffee husk as substrate. The high conidiarnyielding substrates were best productive at their own natural at 6.29 for Vegetablernwastes, 6.63 for Wheat bran and 5.4 for Tea waste pH values. Vegetable wastesrnsupported AUBI1, AUBI2, AUMI1, AUMI2 and AUMI3 to produce high conidia yield ofrn4.31 ± 1.18 (107), 5.07 ± 0.76 (107), 3.43 ± 0.30 (107), 1.86 ± 0.16 (107) and 5.17 ± 0.61rn(107) conidia/gram of substrate at optimum incubation period of 21 days. The optimumrnincubation period of the fungal isolates on wheat bran is 3 - 4 weeks under sufficientrnexposure to light. AUBI1, AUBI2 and AUMI1 produce high conidia under exposure tornlight but AUMI2 and AUMI3 at dark condition when cultivated on vegetable wastes.rnTherefore, as successful microbial control of insect pests depends on cheap and largernscale industrial productivity, entomopathogens cultivation on vegetable wastes and wheatrnbran can be attained by SSF system.rnKey words: Beauveria, Metarhizium, Inoculation, Optimization and Conidia

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