Symbiotic And Phenotypic Characterization Of Common Bean ( .phaseolus Vulgaris)- Nodulating Rhizobia From Some Areas Of Southern Ethiopia

Eighteen isolates of common bean rhizobium from Konso (GI), Arbaminch (GII), Sodo (GUI),rnand Zeway- Awassa-Dilla (GIV ) regions in Southern Ethiopia were isolated and characterized byrntheir phenotypic diversity and symbiotic properties on Red Welaita (RW) and Mexican142rn(Ml 42). All isolates were infective on either of one or both Phciseolus vulgaris cultivars Redrnwolaita (RW ) and Mexican 142 (Ml 42) in glasshouse experiment. The cluster analysis groupedrnthe isolates into two major diversity groups based on 80-100% similarity levels (Group I) andrn39%-76%, similarity levels (Group II). Group I contained 78% of the isolates with two clusters larnand lb with presumed similar characters of Rhizobium legwninosarum/Rhizobium etli grouprnexcept AUPR10. Isolates such as AUPR8 from Group II displayed creamy colony appearance onrnPeptone Yeast Extract Agar medium (PY), tolerance to extreme pH, salt, and temperature andrninability to utilize dulcitol and utilization of glycine that resembled with characters of Rhizobiumrntropici. Two isolates, AUPR9 (Group II) and AUPR 10 (Group I), exhibited rough colonyrnappearance on PY that resemble the characteristics of Rhizobium gallicum. There was significantrndifference in shoot dry, nodule fresh and dry weight on both cultivars (P

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