Evaluation Of Rhizobia And Phosphate Solublizing Rhizobacteria For Enhancing Growth And Yield In Phaseolus Vulgaris L. Via Co- Inoculation In Greenhouse And Field
Microbial, Cellular And Molecular Biology Project Topics
The symbiotic nitrogen fixing effectiveness of common bean nodulating rhizobia and phosphaternsolubilizing abilities of rhizospheric bacteria collected from the major growing areas of Ethiopiarnwere evaluated. Soil samples were randomly collected from farmers’ fields. Isolation of rhizobiarnwas conducted using plant infection method in greenhouse whereas the phosphate solubilizingrnrhizobacteria (PSR) were isolated from the soils adhering to the root surfaces of common bean.rnAuthentication, phenotypic characterization and symbiotic efficiency of 76 rhizobial isolatesrnwere determined following standard methods. PSR were screened for solubilization efficiency onrnsolid and liquid media containing different phosphate sources. All the PSR and rhizobia wererntested for other plant growth promoting (PGP) traits. Single- and co-inoculation trials withrnrhizobial and phosphobacterial isolates were conducted under greenhouse and field trials.rnAnalysis of 16S rRNA gene sequences was performed to identify the top two symbioticallyrneffective rhizobial isolates and all the PSR. The rhizobial isolates, except CBR039 and CBR151,rnwere able to reinfect their host plant. The rhizobia displayed diversity in their phenotypicrncharacteristics and formed four clusters with reference strains that include R.phaseoli, R.etli, R.rngardiani and R. tropici. The two rhizobial isolates, CBR052 and CBR141, with high symbioticrnefficiency were identified as Rhizobium etli and Rhizobium sp., respectively. Fifty six PSR werernisolated in this study and identified into 10 genera that include Bacillus, Arthrobacter,rnBrevibacterium, Lycinibacillus, Enterobacter, Acetinobacter, Pseudomonas, Klebsiella, Devosia,rnand Chryseobacterium. The phosphobacteria released as much as 312.7 μg/ml soluble phosphaternfrom tri-calcium phosphate (TCP). As high as 53.2μg/ml IAA production and 51.3% Fusariumrninhibition was recorded in this experiment. Inoculation of common bean with selectedrnphosphobacterial isolates in greenhouse significantly (p