Isolation Identification Of Fusarium Xylarioides (gibberella Xylarioides) From Southern Ethiopia And Its Response To Fungal Biocontrol Agents

Five isolates of Fusarium xylarioides form five coffee growing Weredas in southwesternrnEthiopia were isolated from Arabica coffee trees showing sever symptoms of vascular wilt.rnThese strains were characterized based on vegetative and spore morphology and vegetativerngrowth on different growth media, temperature and pH ranges. These strains produced bothrnmicroconidia and macroconidia on Potato Dextrose Agar (PDA) and Synthetic low-nutrientrnAgar (SNA). Microconidia were unicellular, allantoids and had 0-1 septate. Macroconidiarnwere strongly curved with marked foot cells and had 1-4 septate. Pathogenicity test wasrnperformed for the subsets of isolates by inoculating 20 ml, 40 ml, and 60 ml of sporernsuspensions. Symptoms developed on the 30th day of inoculation concentration wisely. Allrnseedlings died on the 90 day of inoculation. The controls were drenched with equivalentrnamount of distilled water and no symptoms developed until the completion of the study. Inrnvitro evaluation of antagonistic test of dual culture of the test isolates with twornTrichoderma species showed that the radial growth inhibition of the pathogens. The cellrnfree culture filtrates of different concentration also showed the radial growth inhibition ofrnthe test pathogens.rnKey words: Fusarium xylarioides, Trichodrma harzianum, Trichoderma viride,rnBiological control.

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