In vitro antagonistic activity of Trichoderma isolates against coffee wilt disease (CWD), Fusariumrnxylarioides (Gibberella xylarioides) was studied. Out of 32 soil samples and 7 coffee tree partsrncollected from 6 different coffee growing woredas of Jimma Zone, 7 potential/effectivernTrichoderma isolates were isolated and characterized out of 74 fungal cultures. Trichodermarnisolates were characterized based on spore morphology and vegetative growth characterizations onrndifferent growth media, temperature and pH ranges. The most suitable and maximum growth ofrnTrichoderma isolates was obsereved at pH 4.5 and 5.5 in all of the isolates. In vitro evaluation ofrndual culture test was done for each of the seven Trichoderma isolates against F. xylarioides. It wasrnobserved that all of the seven Trichoderma isolates have shown 63.33 to 70.95 percent of mycelialrngrowth inhibition of F. xylarioides after 6 days of incubation at 250C on potato dextrose agarrnmedium. Trichoderma isolate AUT4 was the most effective biocontrol agent which exhibited thernmaximum inhibition of 70.95%. Whereas, the least percent of mycelial growth inhibition 63.33%rnwas obtained by Trichoderma isolate AUT3. In addition to the above, the seven isolates ofrnTrichoderma were then tested for their production of volatile antifungal compounds against F.rnxylarioides. They produced volatile compounds which inhibited the mycelial gorwth of the testrnpathogen from 66 to 80.60%. Trichoderma isolate AUT1 produced the most effective volatilerncompounds which inhibited growth by 80.60%. Therefore, the application of in vitro evaluations ofrnseven Trichoderma isolates has proved to be highly potential/effective in the control of coffee wiltrndiseases (Fusarium xylarioides).rnKey words: Biological Control agents, cofffee wilt disease, Fusarium xylarioides,rnTrichoderma isolates.