Morphological And Molecular Diversity In Durum Wheat (triticum Durumdesf.) Landraces Of North Shewa

Morphological and molecular diversity study and the associated ethnobotanicalrnprofile of durum wheat landraces (farmers' varieties) of North Shewa have beenrnundertaken in this study. Morphological diversity stndy on thirteen durnm wheatrnpopnlations (381 genotypes) ,collected earlier by the Biodiversity Conservation andrnResearch Institute from five awrajas and ten weredas of North Shewa region, wasrnconducted using six heritable qualitative traits. The diversity was estimated usingrnShannon Weaver diversity index (H'). H' was estimated for each population,rnwereda, awraja and altitudinal class and the entire collection. On population basisrnH' is lowest at Basona Warena wereda (0.25 ± 0.08) and highest at Kimbibit Weredarn(0.65 ± 0.16). On awraja basis H' ranged from (0.50 ± 0.03) in Gam Midir to (0.73 ±rn0.10) in Selale. On altitudinal basis, the third altitudinal class (2401-2600 m.a.s.l.) scrnored the highest H' (0.78). The diversity index for the study area is very high (0.79 ±rn0.08). On character basis seed color showed the highest H' (0.97). Only glumernpubescence was conspicuously unique to specific awmja and altitudinal class.rnA high diversity of the crop is also observed from the ethnobotany study. A total ofrntwenty-five different farmers' varieties having different vernacular names werernrecorded. The environmental, social (cultural) and economic factors were identifiedrnby the farmer as factors for maintaining the existing diversity. The differentrnvarieties have important merits in agronomy, disease resistance, traditional usernvalues and so on. Farmers have their own folk taxonomy system for identifying andrnclassifying their varieties.rnThe Randomly Amplified Polymorphic DNA (RAPD) molecular technique with arngluten-coding single primer system with the following sequence,rn{ 5'- ATG AAG ACC TTC CTC GTC TT- 3'}rn{ 5'-AAC GCC GAA TGG CAC ACT A- 3' }rnshowed good demonstration of the inter-population diversity of the thirteenrnpopulations. Except three populations, all the rest showed diffel'ent bandingrnpatterns with different base pair fmgments for the same primer used. Generally thernwhole study confirmed the existence of strikingly high diversity of durum wheat inrnthe study area and underlines the role of the indigenous knowledge of farmers forrnthe existing diversity.rnKey words: Wheat, Triticum dUrlIm, landraces (fanners' varieties), diversity index (H')rnMorphological traits, ethnobotany, RAPD.

Get Full Work

Report copyright infringement or plagiarism