Alkaline Protease Of Alkaliphiles Isolated From Ethiopian Rift Valley Soda- Lakes

A prntcolytic alkali phi lie bacteria was selected out of the bacterial isolates obtained fromrnRiftvallcy soda lakes. The isolate was obtained from lake Chitu water sample. Thernorganism was Gram variable aerobic, rod shaped spore forming, motile bacterium. It hasrnbeen identified to belongs to the genus Bacillus.rnThe growth of the isolate coded as CH-W 1 was observed in the pH and temperaturernranges between 7-l l and 25-40°C respectively. Protease production was observedrnshortly afler inoculation reaching to maximum after 48 h. The crude enzyme had arntemperature optimum of 55 °C and a pH optimum of 9. The enzyme was stable in arnbroad pH range of 8.5- l 0.5 after l hr incubation at 50 ° C . It has a half-life of 30 min.rnat 60°C . The enzyme was slightly activated by CuSO4 . Where as Ba++ ,Zn ++, Co++ ,rnHg++ , Mn ++, Fc+r ,.Na', K'and Ca" had very little.or no effect cin the activity of thernenzyme. The enzyme was strongly inhibited by I mM PMSF sh o,,ing that it belongsrnto the class of serine protease. EDTA at a concentration of IO mM partially inhibitsrnthe activity. This shows the requirement of Ca'' for stability.rnThe organism was efficient in degrading Nug meal (GuizotaAbyssinia) and featherrnwhen used as a sole carbon source.

Get Full Work

Report copyright infringement or plagiarism