Evaluation Of Different Systems Of Cd4 T-cell Percentage Determinations By Using Standard Flow Cytometry For Pediatric Hiv Management

Antiretroviral monitoring relay on %CD4+ T-cell values particularly in pediatrics. The double tube FACSCount system currently in use does not give %CD4+ T-cell value. A single tube FACSCount system that gives %CD4+ T-cell value has been introduced which requires evaluation. These two different systems were evaluated for %CD4+ T-cell measurement by using standard flow cytometery for pediatric HIV management. Blood specimens were collected using K3EDTA tube from Addis Ababa and its surroundings. Back-calculated %CD4+ T-cells obtained from the double tube FACSCount was compared with %CD4+ T-cells obtained directly from dual platform FACSCalibur TritestTM system in 221 HIV infected children (less than 14 years). Furthermore, %CD4+ T-cell determination by the newly introduced single tube single platform FACSCount was compared with that of single platform FACSCalibur TruCOUNTTM system in 184 HIV infected subjects (from 1.2 to 66 years). Correlation between the back-calculated %CD4+ T-cells values and %CD4+ T-cell from the dual platform FACSCalibur TritestTM was good (r = 0.960, p

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