Grape-vine (Vitis vinifera L.) is a perennial deciduous woody vine which isrncultivated all over the world. Conventional breeding of grape vine is impeded byrnlong juvenile periods and inbreeding depression. The objectives of this studyrnwere to establish a protocol for in vitro micropropagation of selected grape vinernvarieties. Preliminary studies were undertaken in order to optimize the durationrnof sterilization of the explants and the culture used to initiate shoots. Shoot tipsrnof variety Chenin blanc was cultured in MS medium supplemented with 0.5rnmg/l after sterilizing in 1% NaOCl to determine the optimum duration ofrnsterilization. For shoot initiation experiment, shoot-tip and nodes of the threernvarieties, namely, Ugni blanc, Chenin blanc and Canonannon were cultured inrnMurashige-Skoog medium supplemented with five different concentrations ofrnBAP including the BAP free. Various experiments were carried out to optimizernshoot multiplication using MS medium supplemented with differentrncombination and concentrations of BAP with or without IBA. To optimize rootrninduction different concentrations of IAA were used. Data such as number ofrnshoots and root, shoot height, root length and node number were recorded andrnanalyzed. Sterilization of explants using 1% of NaOCl was found to be optimumrnfor 7 minutes duration. Cheninblanc showed high percentage of survival ratern(96%) followed by Ugniblanc and Canonannon (88%) at 0.5 mg/l BAP. Amongrnthe different concentrations and combination of PGRs, a maximum meanrnnumber of shoots 7.2, 6.7, 6.1 was achieved at 1mg/l BAP combined with 0.1rnmg/l IBA for Chenin blanc, Canonnanon and Ugni blanc respectively. Thernshoot length and node number of all varieties showed a decreasing trend whenrnthe concentration of BAP was increased, except for Canonannon, in terms ofrnnode number. Number of shoots, node and shoot height showed significantrnvariability (p=0.05) at different level of BAP or BAP combined with IBA. Allrnvarieties induced root on MS medium without IAA or supplemented withrndifferent concentration of IAA. Among these rooting culture, however, MSrnmedium with higher concentration (2 and 4 mg/l) of IAA were found to be thernbest for root induction in all varieties. The plantlets were acclimatized in thernglasshouse and survival percentage was 92% for Chenin blanc followed byrn78.6% and 73.9% for Ugni blanc and Canonannon, respectively. Thus, thernachievements of this study will play a big role in the grape vine culture program.rnKey words: Vitis vinifera L., shoot initiation, root initiation, Cytokinin, Auxin