Effect Of Naphthalene Acetic Acid (naa) On In Vitro Rooting Of Regenerated Microshoots Of Groundnut (arachis Hypogaea L.)

Successful utilization of tissue culture for the improvement and conservation of groundnut will bemade possible only with an efficient shoot proliferation, formation of well-developed root system inmicroshoots, successful acclimatization and final establishment in field. A regeneration protocolensuring a high frequency rooting of microshoots derived from embryonic axes of four groundnutgenotypes was achieved using naphthalene acetic acid (NAA). Microshoots of 3cm length weresubcultured on Murashige and Skoog (MS) medium plus 30% sucrose, supplement with 1.0 or1.5mg/L NAA and compared with a hormone free medium (control). Rooting was significantlyinfluenced by the presence and concentration of NAA. Microshoots subcultured in mediumsupplemented with 1mg/L NAA rooted within 2weeks of subculture, producing the highest numberof roots/plantlet and root-induction frequency. However, increasing the concentration of the auxinsuppressed root length with the hormone free medium having the longest roots. Significantgenotypic effect was also observed, with Samnut 22 having more number of roots/plantlet androot-induction frequency.

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