Background: Infant feeding practice is the important modifiable factor influencing the health ofrnchildren born from HIV-positive mothers. While breastfeeding carries significant health benefitsrnto infants and young children, HIV can be transmitted during breastfeeding from an HIV-rninfected mother to her infant. Breastfeeding may thus be responsible for 1/3 -1/2 of HIVrninfections in infants in African settings.rnObjective: The aim of this study was to assess infant feeding practice and associated factorrnamong HIV positive mothers attending ART service in governmental health institutions of Bahirrndar city.rnMethods: Institution based cross- sectional study was conducted in 4 health institutions whichrnprovides ART service in Bahir dar town from October to June, 2017. A total of 230 mother-rninfant pairs attending ART clinics were selected by systematic random sampling. The data wererncoded, entered and stored in to the computer using Epi Data version 3.1, then cleaned andrnanalyzed by SPSS software version 20. Bivariate and multivariate models were run to assess anyrnrelationship between independent variable and outcome variable.rnResult: The majority 173(75.2%) practiced exclusive breast feeding up to six months of age. 13.9% andrn10.9% of mothers practiced exclusive replacement feeding and mixed feeding, respectively. Inrnmultivariable logistic regressions, mothers counseled about the recommended infant feeding optionsrnduring delivery (AOR (95%CI):3.699(1.00-13.41), ever given any food or fluid before first breastrnmilk (AOR (95%CI):0.015(0.001-0.188) and knowledge on PMTCT and the recommended infantrnfeeding options (AOR (95%CI):0.189(0.042-0.855) were significantly associated with infantrnfeeding practice.rnConclusion and Recommendation: The study revealed that majority mothers experiencedrnexclusive breast feeding (75.2%). Mothers ever given any food/fluid before first breast milk,rncounseled the recommended feeding options during delivery and knowledge on PMTCT and thernrecommended infant feeding options were independent predictors of infant feeding practice.rnAdequate information should be provided to HIV positive mothers to select the best feedingrnoptions for their child.rnKey words: infant feeding practice, HIV positive mothers, and Bahirdar.