Background: Newborn is considered to be tiny and powerless, completely dependent onrnother for life within one minute of birth the normal new born adapts from a dependent fetalrnexistence to an independent one; capable of breathing and carrying on life process. The firstrnhours are crucial because multiple organ system is making the transition from intrauterine tornextra uterine functions. The care given immediately after birth is simple but important.rnObjective: To assess Knowledge and practice of immediate newborn care among Midwivesrnin governmental health facilities at central zone, Tigray regional state, north Ethiopia, fromrnJanuary- June, 2015.rnMethods: An institutional based cross-sectional quantitative descriptive study design wasrnused for this study. Sample size was calculated by using sample correction formula andrnsample size was 150 midwives. In Central zone all woreda health facilities were included inrnthe study. Final study subject were selected using non probability convenience methods. Therndata was collected after pre-tested the questionnaire. Observation method was usedrnstandardized checklist adopted from save the children international followed by interview.rnThe collected data was coded and entered to EPI-Info version 3.5.1 and data was exported tornSPSS version 21.0. Finally, result was presented in texts, graphs and tables.rnResult: Out of 150 samples size 147 were participated in the study making the response raternof 98%. Of these, 59.9% midwives were working in health center, 99.3% were ethnicity ofrnTigray and 79.6% were females. 23.8% respondents were in the age group of 25-29 and thernmean age of respondents was 34.1 years. Among the total participants, 99.3% of midwivesrnwere applied eye ointment to immediately born baby. The general knowledge of participantsrnon immediate newborn care was 17.7% good and 25.2% poor. Regarding immediate newbornrncare practice 52.4% were practiced but remaining 47.6% of respondents were not practiced.rnWork environment was significantly associated with practice of immediate newborn care.rnMidwives working in health center were 82% lower odds of newborn care compared to thosernworking in hospital (p=0.000, OR=0.18 (0.07, 0.43)rnConclusion and Recommendation: Most midwives have received in-service training onrnimmediate newborn care, even though the Study result showed presence of knowledge andrnpractice gap of care given to newborn baby. So it is necessary to strengthen in-servicerntraining given to midwives on immediate newborn care periodically. Further study onrnknowledge and practice of midwives regarding immediate newborn care is recommended.rnKeyword: Knowledge, practice and newborn care