Hptlc-densitometric Determination Of Some Water Soluble Vitamins Present In Pharmaceuticals

A simple, accurate and precise high-performance thin-layer chromatographic (HPTLC)rnmethod has been developed for the determination of vitamin B1, B6 and B12 in tablet andrninjection. Using methanol: ammonium acetate (5M) (10:1; v/v) as mobile phase andrnHPTLC plates (0.2mm thickness) precoated with 60F254 silica gel on glass plate asrnstationary phase. Quantification was carried out densitometrically using a UV detector atrn255, 290 and 361nm. The retardation factors (Rf) of B1, B6 and B12 were 0.20 ± 0.02, 0.40rn± 0.02 and 0.68 ± 0.02 respectively. Calibration curves were polynomial in the rangern250-2050 ng/μl for B1 and B6, 50-1050 ng/μl for B12.rnThe method has been robust for small variation with mobile phase composition, amountrnof mobile phase and time variation before chromatographic development and scanning.rnThe stability of sample solution was studied over eight hours and no additional spot wasrnobserved on the plate. As a result no evidence was observed for the degradation of thernproduct. In addition the proposed method has been compared statistically (t-test and Ftest)rnwith the classical method (HPLC) and there was no difference in terms of accuracyrnof the dosage analysis between the two methods.rnThe analysis result of both synthetic mixture and pharmaceutical samples showed thernquantitative determination of each component accurately in the presence of the otherrncomponents and back ground interference.rnAssay of vitamin B1, B6 and B12 were 99.10 ± 1.37 %, 99.68 ± 1.50 % and 99.42 ± 1.26rn% for vitamin B1, B6 and B12 injection, 99.44 ± 1.30 % and 99.95 ± 1.49 % for vitaminrnB1 and B6 tablet respectively.

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