Diabetes Mellitus (DM) is a metabolic disorder characterized by hyperglycemia, which is usuallyrndue to an absence of insulin, impaired effectiveness of insulin action or tissue insensitivity torninsulin. It can be classified into type I, type II and gestational DM. Its treatment is based on insulinrninjection and oral hypoglycemic agents. However, rural parts of worldwide people with DM relayrnon traditional remedies from plants sources with minimal side effects. This study was conductedrnto evaluate hypoglycemic effect of 80% methanol extract and solvent fractions of Crotonrnmacrostachyus leaves in normal, glucose loaded and Streptozotocin (STZ)-induced hyperglycemicrnmice. 80% methanol extract and different solvent fractions of Croton macrostachyus leaves werernprepared. Sprague-Dawley mice of either sex were selected for the experiments. In this study micernwere grouped into five groups (six mice per group). Hyperglycemia was induced by singlernintraperitoneal injection of STZ (135mg/kg body weight). Preliminary phytochemical screeningrnwas done using standard procedures and acute toxicity study was done as per OECD 425rnguidelines. The results were analyzed using one way ANOVA followed by post hoc test at 5%rnlevel of significance. 80% methanol and solvent fractions of study plants have blood glucosernlowering effect on normoglycemic, glucose loaded and STZ-induced hyperglycemic mice at allrndoses. However, mice that took chloroform fraction at dose of 100 and 200 mg/kg didnâ€™t show anyrnsignificant reduction in blood glucose level on glucose loaded mice. In addition dose-dependentrnreductions in blood glucose levels were observed in 80% methanol extract and solvent fractions.rnPhytochemical screening indicated that alkaloid; flavonoids, tannins, phenols and sapponin werernpresented in leaves extracts. It was also observed that the extracts didnâ€™t have acute toxicity at arnsingle dose of 2 g/kg body weight of mice. So the traditional use of the study plant for thernmanagement of DM can be supported as an alternative herbal supplement with furtherrninvestigation needed to make final conclusion.