Antiproliferative Activities Of Alkaloids From Crinum Abyscinicum Hochst. Exa. Rich Bulb Extract

Cancers are group of diseases that are distinguished by increased division of cells caused byrngenetic changes. Globally, it accounted for 9.6 million deaths in 2015 and is increasing to be arnpublic health burden. Yet the treatment of cancer is mainly through chemotherapy that possessesrnsevere toxicities and facing multiple drug resistance. These problems have urged researchers tornlook for safe and effective chemotherapeutic agents from natural products, especially fromrnplants. Thus, Crinum abyscinicum as one of such plants in Ethiopia that has been usedrntraditionally for treatment of cancer was evaluated for its antiproliferative activity.rnIn vitro antiproliferative activity of the bulb extract of C. abyscinicum was investigated usingrnMTT and resazurine assays on ovarian carcinoma cell line (A2780) and leukemia cell linern(MV4-11) respectively. It is noted that the bulb extract possesses antiproliferative activity withrnGIrn of 8.289 ± 0.33µg/ml and 20.77 ± 0.354µg/ml against leukemia cell line (MV4-11) andrnovarian carcinoma cell line (A2780) respectively. rn50rnFurther analysis of the bulb extract using preparative thin layer chromatography (PTLC) resultedrnin the isolation of two alkaloids. The structure of the alkaloids were characterized as 6hydroxycrinaminernrn(BCA-1) and lycorine (BCA-2) using spectroscopic methods including HRTOF-MS,rnrn1D-NMR (rn1rnH, rn13rnC-NMR and DEPT) and 2D-NMR (HMBC) spectral data, and byrncomparison with reported spectroscopic data for the same compounds. rnAmong the isolated compounds, lycorine displayed antiproliferative activity in a dose-dependentrnmanner against MV4-11 and A2780 cell lines with GIrnof 3.372 ± 0.26733µg/ml and 2.849 ±rn0.139µg/ml respectively. Likewise, 6-hydroxycrinamine exhibited activity with growthrninhibition of 5.323 ± 0.455µg/ml and 2.925 ± 0.769µg/ml against MV4-11 and A2780rnrespectively. The activity observed for the bulb extract as well as isolated compounds of C.rnabyscinicum support the traditional use of the plant against cancer.

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