Malaria continues as major health problem in subtropical region of the world. The resistances ofrnmalaria parasites to available drugs endanger all the good result that has been found. Therefore,rnthe development of novel drug is a must to do job. One of the key sources is traditionalrnmedicinal plant. Prunes persica (L) Bastche is a common fruit and belongs to the familyrnRosaceae (subfamily Prunoideae). The seed extract of Prunes persica (L) Bastche is traditionallyrnused as anti-malarial agent in Shinasha society North West of Ethiopia. However, no scientificrninvestigation has been carried out to substantiate this claim. This study was conducted tornevaluate anti-plasmodial activity of 80% methanol seed extract and solvent fractions of the plantrnPrunes persica (L) Bastche in mice model. To assess the effect, various dose (200,400 and 800rnmg/kg) of the seed extract and butanol (BF), chloroform (CF) and aqueous (AF) fractions ofrncrude extract were administered to Plasmodium berghei infected mice using 4 day suppressiverntest scheme. The parameters, including parasitemia load, survival time, change in body weight,rntemperature and packed cell volume were then determined within 4 days. The result revealed thatrncrude extract of seed has relatively good parasitemia suppression rate 34.59% at 200mg/kg,rn51.75% at 400mg /kg, 76.04% at 800mg/kg, negative control DW10 ml/kg 0 % and positiverncontrol CQ25mg/kg 100%. From the solvent fractions BF scored highest parasitemia suppressionrnrate 36.64% at 200mg/kg, 46.01% at 400mg/kg, 54.96% at 800mg/kg, negative control DW10rnml/kg 0 % and positive control CQ25mg/kg 100%. AF had second highest parasitemiarnsuppuration rate 32.85% at 200mg/kg, 44.56% at 400mg/kg, 50.99% at 800mg/kg, negativerncontrol DW10 ml/kg 0 % and positive control CQ25mg/kg 100% and CF has lowest parasitemiarnsuppuration rate29.49% at 200mg/kg, 34.15% at 400mg/kg, 44.00% at 800mg/kg, negativerncontrol DW10 ml/kg 0 % and positive control CQ25mg/kg 100%. All dose level of the seedrncrude extract prevented loss of weight, elevation in body temperature and anemia on early andrnestablished infection. BF and AF did also reverse reduction in body weight and packed cellrnvolume. Lastly, phyto-chemical screening of the extracts showed the presence of different rnsecondary metabolites such as tannins, alkaloids, anthocyanins, terpenoids, saponins, phenols,rnand flavonoids. In conclusion, the plant has anti-plasmodial activity.