Discopodium penninervum is among the Ethiopian medicinal plants claimed for wound healingrnactivity. However, to date, there is no scientific report on evaluation of its wound healingrnactivity. Thus, this work was initiated to investigate wound healing activity of 80 % methanolrnleaf extract of D.penninervum in mice.rnAcute oral and dermal toxicity test was studied by observing sign of toxicity for oral and dermalrntoxicity. Wound healing activity was investigated using excision and incision wound models byrntopical application of 5% and 10% (w/w) ointments of 80% methanol leaf extract of thernplant.Parameters such as wound contraction and period of epithelialization were determined fromrnexcision wound model, whiletensile strengthwas evaluated fromincision wound model.Inrnaddition,anti-inflammatory, antibacterial and antioxidant activity studies of the extract were alsornperformed.Carrageenan-induced hind paw edema model was usedfor anti-inflammatory study atrna concentration of 100, 200 and 400 mg/kg. Antibacterial activity of the extract was examinedrnusing agar well diffusion technique against several common bacterial strains for woundrninfection. For antioxidant activity test, DPPH (1, 1-diphenyl-2-picrylhydrazyl) free radicalrnscavenging assay was used. In parallel, total flavonoid content of the extract was determined byrnusing aluminum-chloride colorimetric method.rnThe extract and formulated ointments were found to be safe in oral and dermal toxicity studies,rnrespectively. Both 5% and 10% (w/w) ointments exhibited significant (p< 0.001) increase inrnwound contraction and shorter epithelialization periodin excision wound model and, increasedrntensile strength in incision wound model. Plant extract also showed significant (p