Anticonvulsant Activity Of Soxhlet Leaf Extracts Of Ajuga Integrifolia Buch.ham Ex D.don (lamiaceae) In Mice

Pharmacology Project Topics

Get the Complete Project Materials Now! ยป

Epilepsy is one of the most common chronic neurological conditions that affect 70 millionrnpeople in different parts of the world. The leaves of Ajuga integrifolia Buch.ham ex D Donrnhave been used as anti-convulsant remedy in Ethiopian traditional medicine. However, thernevidence supporting this claim is sparse in the literature. This study was conducted to add tornthe existing body of knowledge about the anti-convulsant activity of the plant. To this effectrnsuccessive Soxhlet extraction was performed using n-hexane, ethyl acetate, methanol andrnwater. rnAnti-convulsant activity of the extracts was investigated in both acute (pentylenetetrazole,rnPTZ; and maximal electroshock, MES) and chronic (PTZ kindling) seizure models. For thernacute model, various doses of the extract (100mg/kg, 200mg/kg and 400mg/kg) werernadministered. Positive controls received sodium valproate (200mg/kg) for PTZ andrnphenytoin (25mg/kg) for MES. Distilled water or 2% tween 80 was used for negativerncontrols. Kindling was induced by repeated alternate day intra-peritoneal administration ofrnsub-convulsive dose of PTZ (35 mg/kg) for 13 days and the most active extract (ethylrnacetate) was tested in this model. Parameters including onset of clonus and duration of hindrnlimb tonic extension were recorded. Moreover, total alkaloid, flavonoid and phenol contentsrnof the most active extract were determined. rnTreatment of mice with ethyl acetate extract produced a superior effect among all solventrnextracts in both PTZ and MES model. The mean latency to clonic seizure was significantlyrnincreased (p

Get Full Work

Report copyright infringement or plagiarism

Be the First to Share On Social



1GB data
1GB data

RELATED TOPICS

1GB data
1GB data
Anticonvulsant Activity Of Soxhlet Leaf Extracts Of Ajuga Integrifolia Buch.ham Ex D.don (lamiaceae) In Mice

184