In Vivo Antihypertensive And In Vitro Vasodilatory Effect Of Crude Extracts And Fractions Of Moringa Stenopetala Leaves In Rodents

Moringa stenopetala is an edible plant that has been medicinally claimed as a remedy for treatmentrnof hypertension, diabetes, malaria and stomach pain mainly in southern parts of Ethiopia.rnThe aim of this study was to evaluate in vivo antihypertensive effect in fructose induced hypertensivernmale Wistar rats and in vitro vasodilatory effect in pre-contracted isolated guinea pigsrnthoracic aorta strip.rnRats were randomly divided into groups; three controls (positive, negative and normal controlrngroup) and 12 treatment groups of six male Wistar rats (n=6). Treatment groups were given dailyrncrude extract, fraction and residue (250, 500, and 1000 mg/kg) of M. stenopetala orally with DFructosern(66% w/v ad libitum). Whereas, positive, negative and normal control groups receivedrnCaptopril (20 mg/kg/day with 66% w/v D-Fructose ad libitum), only D-Fructose (66% w/v ad libitum)rnand distilled water ad libitum, respectively for 15 days. On the other hand, for the in vitrornvasodilatory study guinea pig was sacrificed by gentle cervical dislocation and the thoracic aortarnring was removed, cut spirally and mounted in an organ bath containing 37oC maintained Kreb’srnHenseleit physiological solution aerated with carbogen (95% O2 and 5% CO2) for experiment.rnThe study showed that aqueous and 70% ethanol extracts a significant prevention in increment inrnsystolic, mean and diastolic blood pressure in a dose dependent manner compared with negativernand normal controls. The extracts also showed suppression in increment in a total cholestrerol,rnglucose and triglycerides plasma level compared with normal control. In addition, the oral dailyrnadministration of extracts produced a rise in liver enzymes but no effect on kidney metabolitesrncompared with normal control group. All extracts showed a relaxation effect in pre-contractedrnisolated thoracic aorta of guinea pigs in dose dependent manner.rnThe in vivo antihypertensive, antidyslipidemic and in vitro vasodilatory effect was significantlyrnshown with aqueous extract followed by 70% ethanol extract. But the effect was low in experimentalrngroups that took ethyl acetate fraction and aqueous residue of aqueous extract.rnKeywords: antihypertensive, vasodilatory, in vivo, in vitro, Moringa stenopetala, antidyslipidemic

Get Full Work

Report copyright infringement or plagiarism