Colorectal cancer (CRC) is a malignant tumor recognized as a major cause of morbidity andrnmortality throughout the world. Epidemiological and experimental studies unveiled thernimportance of compounds derived from plants in reducing the risk of CRC. Recent metaanalysesrndemonstrate inverse associations between coffee intake and CRC. The aim of thernpresent study was to evaluate chemopreventive potential of Coffee arabica in DMH-inducedrncolorectal carcinogenesis in the rat model through a two-phase study (initiation and postinitiation).rnThirty five female wistar rats were divided into seven equal groups. Rats in group IVIIrnexcept group VI (received normal saline alone) were given freshly prepared DMH (20 mg/kgrnbody weight, ip) in normal saline and pH adjusted (7.0) in 1mM NaOH, once a week for 5rnweeks. Groups II and III were received additional oral dose of Coffee arabica (20 mg/kg and 40rnmg/kg body weight, respectively) in the initiation phase. Group IV and V were received the samerndose of Coffee arabica given in group II and III, respectively) in the post-initiation phase. GrouprnVII was received low dose aspirin orally. After 10 weeks of treatment period, blood wasrnwithdrawn for serum biochemical analysis, then animals were scarified and their colons werernsubjected to macroscopic and microscopic studies. Taken together, the result of this studyrnshowed that well characterized preneoplastic features such as multiple plaque lesions, aberrantrncrypts and aberrant crypt foci were significantly found in the DMH alone treated group. Thernnumbers were significantly reduced in DMH followed by Coffee arabica or Aspirin treatedrngroups. Histologically different degree of dysplasia and hyperplasia observed in DMH treatedrngroup. The simultaneous administration of DMH and Coffee arabica or Aspirin reduced thesernfeatures. In addition, our results showed that an appreciable counteracts effect of Coffee arabicarnon serum biomarkers and body weights observed in DMH alone treated rats. The results of thisrnstudy surmise that the effects of Coffee arabica may be due to the presence of phenolicrncompounds which have antioxidant activities. It brings suppression of cellular proliferation,rninhibits lipid profile elevation and interferes with serum protein and body weight declination.rnFurthermore, this study was provided that Coffee arabica treatment, in both phases, tended tornconsiderably suppress tumor progression and invasion. The elucidation of the inhibitory effectsrnof Coffee arabica on the progressions and invasion of colorectal preneoplastic lesions is thus ofrngreat importance and will provide promising targets for preventive and therapeutic interventionsrnof CRC.