Reflectance Anisotropy spectroscopy (RAS) is a non-destructive optical probe of surfacesrnthat is capable of operating with in a wide range of environment. In this thesis,rnby applying this optical technique attempt was made to determine the degree ofrnanisotropy of a biaxial Potassium Titanyl phosphate (KTP) crystal. Furthermore, byrnusing rotating polarizer-analyzer method, we have tried to develop a more accuraternand easy to use approach. The method used in this work can be used in situ real-timernmeasurements.The degree of Anisotropy is determined to be 7.4 × 10−3 ± .0001