The aim of this work was to develop micropropagation protocol for the threatened,rnmultipurpose Afromontane tree, Prunus africana (Hook. f.) Kalkman, starting fromrnaseptically grown seedlings. Application of 12% CaOCl2 on propagula for 15 minutes,rnfollowing washing with the common laboratory detergent OMO and rinsing in 70%rnethanol for 2 minutes resulted in seedlings that grew healthily and were free fromrnmicrobial contaminants. Twenty to twenty five-day-old seedlings were used as explants.rnAmong the tested concentrations of growth regulators, 2 mg/l BAP and 0.1 mg/l IBA inrnfull strength MS medium yielded well initiated shoots. Activated charcoal (1g/l) wasrnused for the initiation step, but not required in the subsequent transfers to multiplicationrnmedia, as browning due to phenolic exudates was not a serious threat. Throughout allrnexperiments, an initial pH value of 5.5 was maintained. Maximum shoot multiplicationrn(4.6 shoots per explant) was obtained in response to 1 mg/l BAP without IBA. Thernsufficiently long (3-5 cm) and healthy microshoots that were transferred to halfrnstrength IBA free MS medium with 0.1% activated charcoal gave maximum rootingrnpercentage (60) medium within 20 days. This was significantly higher from otherrncompositions at P < 0.05. The plantlets each with average 11 roots were transferred tornpots, with compost, red soil and sand in a 1: 2: 1 ratio. The pots were placed in arnglasshouse for hardening, were covered with polythene plastic for one week, partiallyrnuncovered for the other one week, and then completely uncovered. All plantletsrnsurvived well.rnKey words/ phrases:- Acclimatization, Culture initiation, Micropropagation,rnPropagules, Prunus africana