From infected sorghum leaves ~ough~ from three differentrnplace, 17 yellol'l coloured bacterial coldmies were isolated. Afterrnhaving conducted hypersensitivi.y, morphological, physiologicalrnand biochemical tests, including pathogenicity test using sorghumrnline W8 1297 as a susceptible host, only one isolate was found tornbe Xanthomonas ~lc~cola. the causal organism of bacterial streakrndisease of sorghum. Six different inoculation techniques wererncompared to achieve an effective method of screening together withrnpathogenicity test. The isolated organism induced typical symptomaticrnresponse when the rub and spray method of inoculation was used.rnUsing the rub and spray method, 31 sorghum lines, 7 from highlandrn(about 2000 metres), 8 from intermediate (1600-1900 metres),rn15 from lowland (800_1600 metres) and sorghum WS 1297 as s controlrnwere examined for resistance to bacterial streak disease caused byrn.!.holcicola. High resistance to the disease was sho"m by sorghumrnlines 80 K 6056, Gambella 1107, 80 ~ 6088, 81 ESIP 21, 81 ESIP 25,rnMelkamash 79, ETS 0601, ETS 4946. Of these 75% are from lowlandrnand 25% are from highland groups. Sorghum lines IS 9294, IS 9302,rnIS 9379, .81 ESIP 29, Dobbs, IS 8686, Muyra white,IS 9923 nnd'thllrncontrol ~S 1297 showed susceptible reactions. Of the susceptiblernsorghum lines 62,5% are from intermediate, 25% from lowland andrn12.5% from highland groups. The reaction of the other lines rangernfrom moderately resistant to moderately susceptible.