Despite the current environmental controversies, flower farms arernbecoming hot areas of investment in Ethiopia. Golden Rose Agro Farm isrnone of the largest, and the pioneer, flower industries in the country. Tornstudy the abundance and distribution of top soil earthworms in relation tornchemical use in the farm, a total of 208 1mx1 m quadrates were markedrnon the ground in 10 randomly selected greenhouses. Similarly, 208rnquadrates were marked in the outside chemical-free farm for comparison.rnSoil was then dug to a depth of 15 cm in all of the marked quadrates andrnhand-sorted to collect top soil earthworms. Eight plastic buckets, eachrnfilled with soil at 15 cm depth, were taken and 15 adult worms originallyrnfrom chemical-free soil were introduced in to each and all were put in therngreenhouses, some under chemical treatment and some free forrncomparison. Worms from the rose farm were also transferred to chemicalfreernsoil and changes were observed after 20 days. Earthworms wererntaxonomically identified to genus level using taxonomic keys. There wasrnstrong statistically significant variation in abundance of both adult andrnjuvenile top soil earthworms between the two farms (P < 0.01, Î±=0.05). Outrnof the 75 adult worms introduced into the chemical-treated buckets, 98.7%rn(74) were dead, and in the Chemical-free buckets, out of the 45 wormsrnoriginally introduced, about 95.6% (38 adult and 5 juveniles) werernrecaptured. About four genera of earthworms were identified in the studyrnarea, but majority of them belong to the genus Eiseniella andrnDendrobaena. Seasonal variation in number and distribution ofrnearthworms was also observed in the study. Chemical use in Golden RosernAgrofarm strongly affected abundance and distribution of top soilrnearthworms in the area