Invitro Study Of The Effects Of Coffee And Thyme On Isolated Airway Smooth Muscle Tissue Of Guinea Pigs

BACKGROUND: Coffea arabica and Thymus schimperi are plants claimed in traditionalrnmedicine to possess numerous beneficial/healing effects including therapeutics in asthma due tornairway smooth muscle relaxant properties. The objective of this study was to investigate thernbronchodilator effects of these plants crude extracts on guinea pig trachea smooth muscles.rnMETHODS: Isolated guinea-pig trachea rings were used to study the effects of hydroalcholic andrnn-hexane crude extracts of the beans of Coffea arabica and the leaves of Thymus schimperi inrnvitro in the presence of acetylcholine by using single dosage method of extract administration.rnSalbutamol was used as a standard drug. RESULTS: Investigation of the rneffects hydroalcholic (methanol 80%) and n-hexane crude extractrnof Coffea arabica L. and Thymus schimperi R. on an isolated trachea smooth muscle tissue in thernpresence of Ach (in vitro) yielded an overall contractile response. The contractile responses of thernplant crude extracts in the absence of Ach were clearly evident on the isolated tissue to variablerndegrees depending on type of plant, type of plant extract and amount of dosage administered tornthe tissue. The tissue contractile response to Ach (160ng) alone, which peaked at 1.75mm (meanrnpeak point) from baseline on the polygraph chart, was used as a positive control for contraction.rnIn the presence of the plant extracts (both plants and types of extracts), the contractile response ofrnthe GPT when Ach was added had an amplified (additive) contractile effect except for the 50μgrndose group of n- hexane crude extract of Coffea arabica, which showed a relaxant effect ofrn0.22mm in the presence of Ach and was not of statistical significance. Statistical analysisrnindicated that the hydroalcholic crude extracts of Coffea arabica and Thymus schimperi showedrnsignificant differences in tissue contraction between the 50μg dose and 100 μg, 200 μg, 400 μgrngroup and between the 50μg dose the two dose groups of 150 μg and 200 μg respectively (Pvaluern_ 0.05).rnCONCLUSION: The hydroalcholic (Methanol 80%) crude extracts of both Coffea arabica L. andrnThymus schimperi R. showed a contractile response on the guinea pig trachea rings in vitro whichrnwas statistically significant between the dose groups in the presence of Ach. The results yieldedrnare not in full agreement to the traditional claim of a relaxant effect on the airways.rnKey words: Coffee;Thyme; Smooth Muscle; Guinea Pig Trachea Rings

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