A further usage of peels from orange as natura dyes is presented in this paper. Natural dye isrngenerally applied in an aqueous solution, and requires a mordant to improve the fastness of therndye on the fiber. Because of its good affinity, brilliant colours, nontoxic, non-allergic nature onrntextile materials, easy accessibility and less damaging effect on human health, it can be used inrnfood, pharmaceutical, and textile industries. The aim of this study is to valorize orange peels forrndye synthesis and to evaluate its yield. Orange peel is used as a raw material for the extraction ofrnnatural dye using soxhlet extraction method. Design of experiment for RSM was used to analyzernand optimize the effect of extraction temperature (65Â°C, 72.5Â°C, and 80Â°C), extraction timern(1.5h, 3.25h and 5h) and orange peel to ethanol ratio (0.05g/mL, 0.075g/mL and 0.1g/mL) onrnextraction of natural dye from orange peels. A maximum yield of natural dye of 40.109% wasrnachieved under selected optimal conditions with high value of combined desirability a threevariable,rnrnthree-level Box-Behnken design was used to develop a statistical model. Fromrnexperiment, the yield of natural dye was obtained as 40.106%. FTIR and UV-Visrnspectrophotometric studies were performed on the dye extract. The dyes produced were appliedrnon cotton fabric and Na2CO3 was used for fixing the color on the fabric. Colour dyeing andrntesting were carried out using data color tool analysis (L*, a*, b*) and fastness properties ofrndyed cotton fabric were investigated according to AATCC-61 and crocking ISO 105Ã—12.