The aim of this study was the formulation of herbal shampoo using Ziziphus Spina Christi L. Three herbal shampoos were produced which contain 10%, 15% and 20% of Ziziphus Spina Christi extract as a surfactant. Sample collection, per treatment, preparation, moisture content determination, extraction and optimization, herbal shampoo production, and characterization of the produced shampoo was the major activity of this research. Central composite design was used to determine, and optimum saponin extraction parameters. Extraction temperature (100-121â„ƒ) and time (15-40 min) have been selected as the major study parameters. Extraction time was the most significant parameter that affecting saponin yield. The quadratic model was found to be accurate enough with a predicted R2 of 0.9858 which was in close agreement with the Adjusted R2 of 0.9806. A temperature of 121 â„ƒ and an extraction time of 40 min were found to be the optimum conditions and the validation experiment resulted in an optimum saponin yield about 6.24%. FTIR results show that N-H stretch group at 3450 cm-1, 1411cm-1 for the C=C group, organic nitrates at 1634.5 cm-1, and Câ€“H group at 712.23 cm-1. The evaluation of the physicochemical test was carried out and compared with aloe vera and organza shampoo. It was observed that many characteristics of the formulated shampoos were in the standard range, and no significant differences regarding their stability at different temperatures, times, and all are physically stable. Formulation (F3) showed all of the ideal properties of the shampoo and showed similar properties to the two reference shampoos and was selected as the best formulation. The research is essential in terms of ensuring the production of an alternative form of herbal shampoo with a wide range of cosmetic applications, as well as having a substantial impact on meeting customer needs and reducing the need for foreign currency. Further research is required to determine cleaning effectiveness, conditioning performance, and toxicity test.