Introduction: -Accessibility of a safe and adequate blood transfusion is a challenge worldwidernand even more critical in sub Saharan Africa. 44 % of maternal deaths due to blood loss duringrnchild birth, 20% of maternal and 15 % of child death as a result of anemia in Africa region couldrnbe managed with a well-timed safe blood transfusion. Though the world health organizationrn(WHO) recommends that blood donation by 2%-3% of the country’s population is needed tornmeet a nation’s most basic requirement for blood, in Ethiopia less than 0.5 % of the populationrndonates blood and nationally there are only 10,000 regular blood donors.rnObjective:-The aim of this study was to evaluate the return rate and return determinants amongrnfirst time voluntary blood donors in Ethiopian national blood bank service (ENBBS).rnMethods:-To determine first time voluntary blood donors return rate secondary data reviewingrnwas done. To investigate return determinants facility based unmatched case control study designrnwas implemented. The cases were first time voluntary blood donors returned for donation withinrntwo years since their index donation whereas controls were those who didn’t return. Pretestedrnquestionnaire was used to guide a telephonic interview to collect data from 438 samples thatrnwere selected randomly from the Ethiopian national blood bank service (ENBBS) donor recordrnserver. Filled data was pre coded and entered in Epidata version 3.1 and was exported to STATArnversion 14 for cleaning and analysis. Descriptive summary measures were used to describe levelsrnof exposure in cases and controls and to assess the presence of association and their significancernlevel a bivariate and multivariate logistic regression analysis were used.rnResult:-Reviewing 24,684 first time voluntary blood donor’s record the return rate was found tornbe 37.7% per two years. First time voluntary blood donor return was found more likely to berndetermined by altruism as a motivational factor for an index donation (AOR=1.96, 95% CI: 1.02-rn3.64) , positive perceived donation capability (AOR=7.3, 95% CI: 2.9 -15.04), Being aware ofrnpreviously donated blood volume (AOR=4.8, 95% CI: 2.18 - 8.98).rnConclusion:-Return rate of first time voluntary blood donors in ENBBS was low and return forrnsubsequent donation was found more likely to be determined by donor's altruistic behavior,rnpositive perceived capability and awareness on blood donation process. Therefore working onrnVIIrndonor’s awareness on the donation process and promoting encouraging message on building uprnaltruistic motivation could enhance the return of first time voluntary blood donors.