Background: Despite the increasing population and unsatisfactory decline of neonatalrnmortality rate (NMR) less attention was given to Neonatal Intensive Care Unit (NICU) untilrnrecent years where a little effort is being made to improve the services provided. Currentlyrnthere are 27governmental NICUs; Tikur Anbessa Specialized Teaching Hospital (TASTH) isrnone of these hospitals which serve as the main referral center. Regardless of the few numberrnof NICUs there is complaints on the quality of care provided which might be due to a varietyrnof reasons that are crucial for the survival of the neonates.rnObjectives: To assess the quality of care in TASTH, NICU in Addis Ababa, Ethiopia.rnMethods: Hospital based cross sectional study was used. The study has two categories ofrnparticipants; primarily mother of the neonates who were available in the NICU during therndata collection period and health professionals (n=35) working in the NICU. Sample size ofrnthe study on mothers was calculated using single population formula considering 50%rnprevalence which gives a total 384 participants. The unit was observed using inventoryrnchecklist from WHO Hospital Care for Mothers and Newborns: Quality Assessment andrnImprovement Tool. For the qualitative portion head of the unit (neonatologist), practicingrnneonatologist and the head nurse were included. Structured questionnaire was adopted fromrnWHO Hospital care for mothers and new-born babies: quality assessment and improvementrntool, and they were filled by the date collectors after obtaining an informed consent. Data wasrnanalyzed using SPSS version 20. P value of 0.05 was the cut off point for the level ofrnsignificance. Adjusted odds ratio was used to show strength of association.rnResult: The study found that the quality of care that is provided in the neonatal intensive carernunit in TASTH is inadequate. Overall service satisfaction was found to be 96.4% and 47.1%rnfor the clients and health professionals respectively. Clients whom their neonates shared bedrnwith others were 6.6 times not satisfied than those clients whom didnâ€™t, AOR (95%CI)rn6.612[3.604, 10.109] Health professionals who donâ€™t think that there are a sufficient numberrnof staffs were dissatisfied 3.15 times compared to those who think the number of staff isrnsufficient. AOR (95%CI) 150[0.050, 5.611]rnConclusion and recommendation: Quality administration demands several strategies thus, itrnis fundamental to identify the problems, aiming at implementing effective actions, andrnmonitoring the processes. The findings from this assessment would assist in scaling up ofrnsuch units. More over the presence of national guideline which is specific to NICU would berna key to solve the problems associated with quality of care in NICUs.