Samples of urine and high vaginal swab (H.V.S) were collected from park lane Enugu. The organisms isolated were pure culture of staphylococcus aureus and Escherichia coli

Sensitivity test was carried out with the use of sensitivity disk containing various minimum inhibitory concentration of the different antibiotics.

The result obtained shaved that gentamicn was more effective followed by chloramphenicol while some organisms shaved resistant to streptomycin.

LIST OF TABLE

TABLE 1 NUTRIENT AGAR

TABLE 2 BLOOD AGAR

TABLE 3: ANTIBIOTICS AGAINST SYAPHYLOCOCCUS AUREUS

TABLE 4: ANTIBIOTIC AGAINST ESCHERICHIA COLI

LIST OF FIGURES

FIGURE 1: STREPTOMYCIN

FIGURE 2: CHLORAMPHENICOL

FIGURE3: SENSITIVITY TEST ON STAPHYLOCOCCUS AUREUS

FIGURE 4 SENSITIVITY TEST ON ESCHERICHIA COLI

FIGURE 5: STAPHYLOCOCCUS AUREUS AGAINST ANTIBIOTICS

FIGURE 6: ESCHERICHIA COLI AGAINST ANTIBIOTICS

TABLE OF CONTEANT

Title page

Certification

Dedication

Acknowledgement

Abstract

List of tables

List of figures

Table of contents

CHAPTER ONE

1.0 Introduction

1.1 Historical background

1.2 Aims and objective

1.3 Hypothesis

1.4 Statement of problem

1.5 Scope of study

1.6 Limitation of study

CHAPTER TWO

2.0 Literature review

3.1 Procurement of antibiotics

3.1.1 streptomycin

2.1.2 Chloramphenicol

2.1.3 Gentamicin

2.2 Antimicrobial activity in vitro

2.3 Antimicrobial activity in vivo

2.4 Resistance to antimicrobial drugs

2.5 Factors affecting antimicrobial activity

2.6 Clinical use of antibiotics

2.7 Characteristic of antibiotics

CHAPTER THREE

3.0 Materials and method

3.1 Sample collection

3.1.1 Media composition

3.1.2 Direction for the preparation of nutrient agar

3.1.3 Direction for the preparation of blood agar

3.1.4 Material used for the prewritten of media

CHAPTER FOUR

4.0 Isolation and identification procedures

4.1.1 Isolation and identification of staphylococcus aureus

4.1.2 Isolation and identification of escherichia coli

4.2 Sensitivity test

CHAPTER FIVE

5.0 RECOMMENDATION

5.1 CONCLUSION

REFERENCES

CHAPTER ONE

INTRODUCTION

1.1 HISTORICAL BACK GROUND

Antibiotics are chemicals when the chemical are put into the body, they stop the growth of kinds of germs. They help the body to fight diseases. More than 3,000 years ago ancient people stumbled over the discovery that some moulds could be used as a cure. The egyptians, the chinese, and indians of central American would use mold to treat rashes and infected would. At that time they didn’t understand either diseases or treatment. As time went on, people began to gain some insight of disease. In the 1860 Louis Pasteur Shaw that many disease were caused it bacteria. Later he discovered that we may be able to fight germ and other microbes. It was two German doctors, who were first to make an effective medication form microbes. Kudo if and Emmerich and Oscar has conducted their experiment in the 1890. They proved that germ that would for another. All the men did was to take the germ from infected bandages and grow then in a test tube. They would then isolate a particular germ that caused green in factions in open would. This germs was bacteria called Bacillus pyoicyaneus. They put then into another test tube containing other type of bacteria, it was then it happened that the bacillus pyocyaneus wiped out the other disease germ. The germs that was killed were those that