This work is aimed at determining the incidence of bacterial injections such as Salmonella and Escherichia coli which cause health hazards to the poultry in their feeds. Sample feed were obtained from commercial mash operation/ pellet mill operation at New heaven and top feed at Ogbete main market Enugu. The samples were screened for total aerobic bacteria count, total coliform count and the characterization of the bacteria isolates carried out while indole test and citrate test were used to confirm the presence of Escherichia coli. The confirmation of salmonella isolates on Mac conkey agar was also done.

Title page

Certification

Dedication

Acknowledgement

Abstract

Tables of contents

List of tables

CHAPATER ONE

1.0 Introduction

1.1 Statement of problem

1.2 Aims and objectives of the study

1.3 Limitation of study

1.4 Hypotheses

CHAPTER TWO

2.0 Literature review

2.1 Nutritional value

2.2 Economic importance

2.3 Disease cause by microorganisms

2.4 Livestock (poultry) feeds manufacturer

2.5 Nutrient /feed requirements

2.6 Nutritive values of eggs

2.7 Standard of feed quality

2.8 Types of poultry feeds & march ingredients

2.9 Quality control of feeds

CHAPTER THREE

3.0 Materials and method

3.1 Sample collection

3.1.1 Microbiological analysis

3.2 Characterization of bacateria

3.2.1 Gram’s staining reaction

3.2.2 Indole test

3.2.3 Methyl red (Mr.) voges proskaver (vp) test

3.2.4 Citrate test

3.2.5 Carbohydrate fermentation

3.2.6 Motility test

3.2.7 Oxidase test

CHAPTER FOUR

4.0 Result

4.1 Total aerobic bacterial count

4.2 Coliform bacterial count

4.3 Characterization of coliform isolates

4.4 Characterization of salmonella isolates

CHAPTER FIVE

Discussion

Conclusion

Recommendation

References

CHAPTER SIX

6.0 Appendixes

6.1 Hard wares

6.2 Glass wares

6.3 Reagent

6.4 Composition for bacteriological culture

LIST OF TABLES

Table I: Total aerobic bacterial counts 55

Table ii: Coliform bacterial counts 56

Table iii Biochemical characteristics of

Isolates (Escherichia coli) 57

Table iv: Biochemical characteristics of

isolates (Salmonella) 58

LIST OF FIGURES

Figure I: chemical composition of the various

constituents of the raw materials used 23

Figure II: Flow chart of poultry feed 24

Figure III: Energy, protein and Amino acid

requirement of chicken 27

Figure iv: March Ingredient 36

CHAPTER ONE

2.0 INTRODUCTION

The term poultry used in agriculture generally refers to all domesticated birds kepts for egg or meat production. These includes chickens (domestic fowls), Grallus domestics, turkeys, ducks and geese. This project will deal largely with the chicken which are the most common domestic fowl. The species of poultry are aptable or can survive in different environments therefore, they are widely distributed in the world, with great increase in population, the demand for goods especially protein food become acute. To meet the demand for animal protein and its development, rapid multiplying ones becomes very necessary. Poultry has so many advantages over other domesticated animal whose production is very much hindered by lack of money, high temperature, disease and lack of good at certain periods of the year.

The diseases of poultry is like the disease of other animals may be caused by: a. pathogenic organisms like bacteria and viruses, protozoa, worms and some members of arthropods

b. Nutritional deficiency, (c) From wound or cannibalism

The following diseases are usually associated with the fowls locally

1. New castle disease

2. Chronic respiratory diseases

3. Fowl typhoid

4. Coccidiosis