The antibacterial activity of three types of medicated soaps on Staphylococcus aureus isolated from wound infections samples were collected from fifty individual with wound infection within the age range of 9-73 years. Swabsticks were used to collect specimens from wound infection. Each swabstick was streaked separately over plates of nutrient and MacConkey agar and later incubated at 37⁰C. Out of the fifty (50) individuals sampled 25(50%) were mostly infected with Staphylococcus aureus followed by Pseudomonas aeruginsa (22%) Staphylococcus epidermis (10%) least by Escherichia coli 6 (12%). The organisms occur within the age range 9-13 years with 8 (16%) followed by 14-18 years 4 (8%) next by the age range of 19-23 years 3(6%) followed by 24-28 years with 2 (4%) and the least in the age range are 29-33, 34-38, 39-43, 44-48, 49-53, 54-58, 64-68 with 1(2%) each while there was no isolation in the age range 69-73. The study also revealed that all the Staphylococcus aureus isolated were sensitive to the three medicated soaps. The zone of inhibition was highest in Asepso medicated soap with 12.92mm followed by Tetmosol medicated soap with 11.2mm and least in Carat medicated soap with 10.56mm. Also the Staphylococcus aureus isolated were also sensitive to the commercial antibiotics used as couplers zones of inhibition was highest in ampiclox with 12.8mm followed by flucloxacillin with 12.04mm and the least in penicillin with 10.4mm. There was a close relationship in the zones of inhibition between the three medicated soaps and the commercial antibiotics used as controls. The result showed that medicated soaps were effective against Staphylococcus aureus involved in wound infection in humans and are comparable to commercial antibiotics. Therefore, medicated soap can be used in cleaning the skin particularly in the affected part of the skin during the time one has hurt or cut on the skin.

CHAPTER ONE

1.0 INTRODUCTION

According to pelage et al, (1986) antibacterial activity is the ability to either destroy bacteria or inhibit their growth. This is significant with respects to the human body in preventing sepsis and skin infections. Also Derland (1981) states that medicated soap have the germicidal substance like chloroxyhlenol potassium mercuric iodide, trichlorocarbanlide etc. incorporated into them, in order to enhance their antibacterial activity. These germicide substance are normally added in a specified amount and percentage of the substance used are always stated on the soapcase or inside the leaflet which certains the information on how to use the soap for various purposes.

Anon (1964) states that soap may be defined as a chemical compound resulting from the interaction of fatty acids oil and caustic soda (alkali) possessing the characteristic soap like properties of detergents, surface tension lowering wetting and emulsifying power and gel formation. All fatty oils and fats are mixtures of glycoside compounds (erters) of trihydric alcohol, glycosides and some fatty acids such as plasmatic acid the chemistry of soap manufacturing may be expressed the following equation which is know as saponification (Anon (1964).

H₂CocoG₅H₃₁)₃ + Na 0H Naoco₁₅H₃₁ (_zH_{31 + c}3_H5_(oH)3

Palliation caustic sodium palurtate glycerion

Soda

Amon (1964) further explain that a bactericidal soap cernirts existentially of the following 0.1 to 3% weight based on the total weight of the soap.

0 to 10% by weight based on the total weight of the soap in a compound of formular

R-CO-N₁-CH₂COOH

CH₃

Here R represents an alkyl or alkaline group of 8 to carbon atoms.

According to Johnson (1978), antibacterial soap may include 2,4,4,trichloro 2,2 hydroxydiphenyl in an amount 0.05 to 5% by weight and at least one phosphorus oxyacid. Phosphorus oxyacid salt phosphoric ester is represented by (R₁(oCH,CH₂)

R₂

Where R₁ in (₈-20 alkyl, R₂ is hydrogen or methyl and is (1-10). R₁ is the same group as R₂or H alkali metal and n is H or alkali metal. Such a soap has a wide range of antibacterial activities and marked resistance of discoloration upon exposure to sunlight (Kaw,1981) another invention relates to an antimicrobial composition consisting of ammoniated zinc sulphate optionally formulated into sufficient formulation and toilet bars. Medicated soap incorporate in their composition germicidal agent which include hexachlorophene mercuric which trichoran, trichlorocarbaucide .

Sykes (1958) described sterilization as the complete destruction of all living matters. In medical sense, it is often used in a restricted sense to refer to the destruction of pathogenic organisms only.

According to William (1979), wound is defined as disruption of cellular and anatomic continuity while its healing is the restoration of continuity. That biological procen can only be accomplished by regeneration, cell proliferation and collage production which can be alleviated washing the would surface especially with medicated soap which due to its concert of pherolic compound help in keeping off organisms like Staphylococcus aureus Escherichia coli and Pseudomonas aeruginosa always from the would to a certain stage. Wound can also result when the operative barrier of the skin is breached by traumatic invasion or whether it is caused by trauma or internationally by surgery. The open area is susceptible to microbial invasion and once a wound has become infected pus form in the injured area resulting to wound abbess.

Baker et al described antisepsis as the most convenient way of preventing infection usually by inhibiting the growth of bacteria. And most disinfectant when suitably diluted have the antiseptic action. Different method are employed for the destruction of bacteria or for getting rid of them and those method can be conveniently divided into chemical physical and mechanical methods.

Baker et al (1985) explained that chemical agent function as a sterilizing agent by the following lethal mechanisms

1. Disruption of the cell membranes

2. Interfering with orgymatic systems of the organisms enzyme poison)

3. Coagulation of protein

4. Oxidation

1.1 AIMS AND OBJECTIVES OF THE STUDY

1. To isolate Staphylococcus aureus from would infection

2. To determine the antibacterial activity of three medicated soap on Staphylococcus aureus isolated from wound infection.

1.2 STATEMENT OF PROBLEM

Statement of the problems in this work shows that medicated soaps have germidical substances like chloroxylenol pothaium, mercuric widide trichlorocarberihide etc. incorporated into them in order to creat their antibacterial activity. It is therefore necessary to investigate the antibacterial activity of three medicated soap (Asepso Tetmosol and Carat) on Staphylococcus aureus isolated from wound infection.

1.3 HYPOTHESES

Medicated soaps have antibacterial activity

Medicated soaps have no antibacterial activity.