A medical gas pipeline system is a standardized method by World Health Organizationrn(WHO) to deliver different medical gases from a centralized source of supply to the requiredrnterminal units using a carefully and well-designed pipeline distribution system. Since the expensiverncost of an exclusive system designing software, most commercial companies use customized andrnunofficial Excel spreadsheet templates. This made the research area very demanding and unwieldyrnfor further investigation. Hence powerful & explanatory MATLAB program is introduced for anrnoptimized system design analysis. This thesis follows a conservative approach of the HealthrnTechnical Memorandum (HTM 02-01, UK) part A: Medical gas pipeline system design manualrnfor the analysis. The thesis aimed to provide the safe and reliable Medical Gas Pipeline DistributionrnSystem design simulation platform using MATLAB software for the following 6 major &rnfrequently used medical gases. Namely: Oxygen (O2), Medical Air (MA), Medical Vacuum (MV),rnSurgical Air (SA), Nitrous Oxide (N2O), Anesthetic Gas (AG). The system design analysis solvesrnthe pipeline distribution layout name coding for the main pipes and its sub branches, flowraternanalysis, determination of the most remote pipeline and its pressure drop analysis. The manualrnsystem design analysis is also introduced and compared with the MATLAB analysis result for thernsample two floors building. Therefore, the MATLAB program can analyze any number of floorsrnwith unlimited number of pipe arrangements in a well-organized design procedure. therefore, itâ€™srna generalized design platform for designing the medical gas pipeline distribution system for anyrnhealthcare centers.