Cross sectional study and isolation of M. gallisepticum was conducted from November 2006 tornApril 2007 in commercial and backyard chickens in East Shewa, Ethiopia. A total of 514 serarnwere collected for sero-prevalence study, of which 187 were from commercial chickens and 327rnfrom backyard chickens. All sera were tested using an indirect ELISA to detect antibodies to M.rngallisepticum infection. This preliminary study was conducted to determine the seroprevalence ofrnM. gallisepticum infection, to assess epidemiological risk factors and to isolate M. gallisepticum.rnThe overall sero-prevalence of M. gallisepticum infection was 49.4% (254/514). Out of this,rn64.5% (211/327) in backyard chickens and 23% (43/187) in commercial chickens and this wasrnstatistically significant difference (p