The present stud} wa~ conducted rrom August 2004 to Apnl 2005 L1h Iht: ObJcclle~ IIfrndctemumng. the prevalence, extent and d istribution 0 dourine III horses and IOCSlIgalingrnoccurrence of cllmeal cases 3S well 3S carner st:ltes of donkey .. and mules III the Arst- B:llcrnIllghla nds. At the same time a longLtudmal study was conducted to assess the efficacy of thernIsomet::mudlUlll chlonde ( ISMM) Hnd DlITu nazenc acetumte (I)I~ I ) III Iht treatment of chmcalrncases of dou rine. An IIlvcs ligalion was also conducted encom p a~Slllg cluesttonmure nndrnserological survey on the presence of dOUrine in adjacent geographic"l areas of the Ar or any other haemopara~l1c~rncould be detected in all cxanllned Glemsa stained smears (th in blood. gell ital di sc harge and ti~l;uCrnfluids) as well as In blood samples by mil CT. All the puppIes Inoculated lth blood samrk~.rngellll.tl washes and oedcmalOUS nUids remained parasl1ologicall) Ilegaue. Diffe rentrncharactcri stic clinical signs of dourinc were observed in clilllcally ~Ic k horses of both sexes. Thernprominent clinical signs observed as genital fom) of the disease mclude vagmal dl:.chargc mallll~rnof mucopurulent type "ilh foul odour. oedema lind presence of deplgmcn ted ..,cars 0 ('1' till'rno,:)'lernal genita lia, oc,icmu of Ihe