Evaluation Of Semen Parameters In Ethiopian Indigenous Bulls Kept At Kaliti Artificial Insemination Center Addis Ababa Ethiopia

The semen of six indigenous breeds bulls (Barca, Boran, Horro and Sheko) which were keptrnat the National Artificial Insemination Center (NAIC), Kaliti, Addis Ababa, Ethiopia werernevaluated for physico-morphological parameters (volume, color, mass and individual motility,rnconcentration, total count, percentage live and morphologically normal, total morphologicallyrnnormal and viable number), biochemical parameters (GOT, GPT, ACP, ALP, total protein)rnand hormonal assay (testosterone). Semen was collected for a thirteen week period from sixrnindigenous bulls kept at the AI center. The collection was done once per week using anrnartificial vagina and semen samples were subjected to normal physico-morphologicalrncharacterization and seminal plasma was separated and preserved at -20 0C until analysis.rnThe overall mean (SE) value for the indigenous bulls in the semen characteristics studiedrnwere volume 4.84 (0.20) ml, mass motility 3.15 (0.11), individual motility 68.72 (1.37)rnpercent, concentration 1.54 (0.07) billion/ml, total count 7.35 (0.47) billion/ejaculate, viablernnumber 5.10 (0.33) billion/ejaculate, total morphologically normal number 7.12 (0.45)rnbillion/ejaculate, live percentage 79.73 (0.65), percent morphologic normal 94.70 (0.38),rnpercent normal acrosome 96.99 (0.60), GOT 1530.91 (60.15) U/L, GPT 131.99 (9.36) U/L,rnALP 3333.98 (608.84) U/L, ACP 8003.68 (716.06) U/L, total protein 7.38 (0.26) gm/dL,rntestosterone 2.84 (0.3) ng/ml, head abnormality 1.87 (0.15) %, mid-piece (body) abnormalityrn1.92 (0.22) %, tail abnormality 1.50 (0.18) %, total abnormality 5.29 (0.38) %, majorrnabnormality 2.25 (0.19) %, minor abnormality 3.04 (0.28) %, acrosome defect 3.0 (0.60) %,rnnarrow at base 0.37 (0.09) %, abnormal contour 0.12 (0.04) %, undeveloped form 0.74 (0.15)rn%, detached abnormal 0.21 (0.09) %, pear shaped 0.05 (0.02) %, small abnormal 0.05 (0.03)rn%, abaxial attachment 0.57 (0.11) %, other head abnormality 0.07 (0.03) %, spermatozoarnhead length 9.18 (0.03) μm, head breadth 4.61 (0.01) μm, mid-piece length 13.61 (0.02) μm,rntail length 47.56 (0.1) μm.rnThe semen physico-morphologic and seminal plasma biochemical parameters observed in thisrnstudy for the Ethiopian indigenous bulls are comparable to normal fertile bulls reportedrnelsewhere based on the evaluation of fresh semen parameters.rnKey words: Ethiopia/NAIC/Indigenous breeds/physico-morphologicalrnparameters/biochemical parameters/semen/spermatozoa

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