Mosquito borne diseases poses an immense veterinary and public health concern and arernmajor impediments in the path of socio-economic development. West Nile Virus is arnwidely distributed mosquito-borne Flavivirus. Environmental management strategies thatrnreduce or eliminate mosquito breeding sites combined with improved personal preventionrnstrategies can help to significantly reduce transmission of the virus. The main aim of thisrnstudy was to detect West Nile Virus in mosquitoes collected from different areas in thernRift Valley of Ethiopia and assess the knowledge, attitude and practices of therncommunity living in the study areas. A cross-sectional study was conducted to collectrnmosquitoes and questionnaire survey in the selected areas. A total of 2,322 mosquitoesrnwere collected, identified to genus level and pooled into 38 groups of approximately 20rnto 25 mosquitoes based on their genera, sex and collection site. The mosquito pools werernhomogenized and the RNA was extracted. WNV genome detection in mosquito poolsrnwas conducted using one-step RT-PCR followed by gel electrophoresis visualization.rnPretested questionnaire was used for interviewing randomly selected households in whichrna total of 288 respondents were participated. Descriptive statistical data analysis testsrnwere used. None of the mosquito pools was positive for WNV genome. Majority (94.4%)rnof the participants know mosquitoes can transmit diseases from humans to humans whilernonly 10.1% know mosquitoes can transmit diseases between human and animals. All ofrnthe participants consider mosquitoes as a threat to the community and 93.8% believedrncontrolling mosquitoes can help in prevention of mosquito borne diseases. Ninety threernpoint one percent of the participants were practicing mosquito control measures in thernpast times. The negative result for WNV genome detection doesnâ€™t mean a total absencernof the virus in the study area, perhaps due to seasonal, feeding and mosquito abundancernfactors. The awareness, attitude and practices of the community regarding mosquitorncontrol and prevention is satisfactory though there are some gaps.