Comparison Of Conception Rate Of Frozen Semen From Two Extenders And Methods Of Early Pregnancy Diagnosis Using Ultrasonography And Progesterone Analysis In Dairy Cattle

Semen extenders used in Ethiopia are imported and expensive. Pregnancy detection is mostlyrnthrough rectal palpation. This study was designed to compare conception rates of frozen semenrnprepared by commercial (Bioxcell) and homemade (Tris egg yolk) extenders. Further, evaluaternadvanced methods of early pregnancy diagnosis (18th to 22nd days post AI) using Progesteronernanalysis and Ultrasonography. Post-thaw semen viability, forward motility, live percent andrnmorphological abnormality were 67±2.6%; 43.59±3.7%; 47.22±1.8% and 42.12±1.26% for TEYrnpreserved semen, respectively; while the same parameters were 68.11±2.6%; 40.83±4.0%;rn46.52±1.7% and 41.64±1.1% for Bioxcell preserved semen, respectively. HOS test was higherrnin TEY (8.87±0.72) extended semen than that of BioXcell extended semen (4.45±1.20%). A totalrn120 animals were included for both study, 48 animals for conception rate of semen extenders (14rnheifers and 34 cows) were equally grouped and inseminated (24 by Bioxcell – G-I and 24 by Trisrnegg yolk – G-II). First service conception rate was 50% for G-I and 70.8% for G-II. Thoughrnconception rate was higher in G-II, there were statistically non significant but higher valuesrnbetween the groups. Nevertheless, conception rate was higher for both heifers and cows withinrnG-II (71.42% and 70.58% in G-II compared to 42.85% and 52.94% in G-I). From a total of 72rnanimals inseminated for early pregnancy detection, pregnancy was confirmed on Days 18th andrn22nd by RMPT and serum P4 analysis 42(58.4%) and 37(51.4%) respectively, and on Day 28thrnUltrasonography 32 (44.5%) post AI. Although pregnancy detection with RMPT was statisticallyrnnon significant but the values was higher between the tests. RMPT was the most sensitivern(88.9%) but less specific (72.2%) taking Ultrasonography as a gold standard. Pregnancy laterrnperformed by transrectal palpation on Day 40 post AI showed 27 (37%) indicating the presencernof corpus luteum and early embryonic mortality. RMPT was found to be relatively cheaper, andrneasier to apply under field condition. Results comparable to ultrasonography can be found if therntest is repeated 7-8 days later. In conclusion, semen extenders can be locally prepared to mitigaternthe cost commercial extenders with even better conception results. Diagnosis of pregnancy canrnalso be made at an earlier stage using a RMPT under the field conditions of Ethiopia.

Get Full Work

Report copyright infringement or plagiarism