A cross sectional study was conducted to determine prevalence, assess risk factors associated with fasciolosis and evaluate economic losses in four selected areas of Tigray Regional State, during September 2007 to May 2008. A total of 1736 domestic ruminants comprising of 1015 cattle, 526 sheep, 167 goats and 28 camel were subjected to coprological investigation and 236 slaughtered animals were included for post mortem survey. Based on coprological investigation the overall prevalence of fasciolosis was 25.3%, 35.7%, 11.4% and 3.6% in cattle, sheep, goats and camels, respectively. In the slaughterhouses survey the prevalence of fasciolosis was 33.1%, 37.2%, and 17.6% in cattle, sheep and goats, respectively. High prevalence was recorded in sheep (37.2%) and lowest in goats (17.6%). Statistical significant differences was observed in prevalence among the species (p