Serial ultrasonographic study was conducted on nine Jennies aged 5-14 years from December 2007 to April, 2008 with the objective of characterizing ovarian follicular activities and estrus manifestations under controlled management. The jennies were housed, fed on hay and concentrate, regularly watered and were not working during the study. Ovarian follicular activity was determined from number and size distribution of follicles, length of interovulatory interval, growth rate of preovulatory follicles, diameter of follicles at the onset of estrus and incidence of ovulation. Estrus manifestations were characterized using length of estrus and estrous cycle. The mean (± SD) number of follicle detected per ovary was 5.45 ± 2.25(range 1 -16) with sizes ranging from 2.88mm to 44mm.The mean (± SD) size of follicle encountered at the onset of estrus was 25.9 ± 3.7 mm (range 20.9 – 34.4) while that of the preovulatory follicles at -1 day before ovulation was 36.81 ± 3.78mm. The mean (± SD) interovulatory interval, estrus and estrous cycle length were 25.4 ± 3.6; 7.85± 2.99 and 24.22 ± 7.43 days respectively. The mean (± SD) growth rate of the preovulatory follicle after day of divergence, which fell on -8 day before ovulation was 1.89 ±0.33mm/day (P