Capripoxviruses cause a severe problem and great economic losses in sheep, goats andrncattle rearing countries of the world. In endemic regions of Africa, Middle East and inrnlarge parts of Asia, Capripoxviruses causesâ€™ severe problem to domestic ruminants with arndevastating effect. This disease was endemic in Ethiopia and cause significant economicrnloss through damage to hide and skin, decreased milk yield, weight loss and exposure tornother diseases. The effective and rapid laboratory diagnostic techniques needed to detectrntentative clinical cases of Capripoxviruses.rnIn this study, 45 skin scrap, 27 buffy coat and 14 nasal swabs samples were collected fromrnOromia, Amhara, SNNPR, Tigray and Addis Ababa region with active outbreak cases ofrnsuspected sheep, goats and cattle to detect and to genotype the Capripoxviruses circulatingrnin Ethiopia.rnA total of 86 field samples were collected from sheep, goats and cattle. The overallrnpercentages of positive samples using conventional PCR from skin scrap, nasal swab andrnbuffycoat in cattle were 85.36% (35/41), 69.23% (9/13) and (0/27) respectively. Thernconventional PCR results in cattle also indicated that detection of LSDV is high in the skinrnscrap than in nasal swab. In real time PCR detection for skin scrap, nasal swab andrnbuffycoat were 92.68% (38/41), 69.23% (9/13), and 70.37% (19/27) positive for LSDVrnrespectively. Real time PCR was more sensitive to detect buffycoat samples thanrnconventional PCR. In small ruminants all samples were 100% positive by conventionalrnand real time PCR in skin scrap and nasal swab samples.rnIn current study, high resolution melting (HRM) assay also used for genotypingrnCapripoxvirus samples, in which LSDV differentiated from cattle and GTPV from sheeprnand goats.rnTherefore, early detection and genotyping method for Capripoxviruses will contributernsignificantly for designing control strategy.